ABSTRACT
OBJECTIVE:To optimize and improve the quality standards of Daziran wuhua yin. METHODS:TLC was used for the qualitative identification of Cichorii Herba,Glycyrrhizae Radix et Rhizoma and Gossampinus malabarica. HPLC was used for the contents determination of chlorogenic acid and tectoridin. The column was Diamonsil C18 with mobile phase of acetoni-trile-0.4% phosphoric acid(pH adjusted with to 2.05 with triethylamine)(10∶90,V/V,chlorogenic acid or 18∶82,V/V,tectoridin) at a flow rate of 1 ml/min,the detection wavelength was 327 nm(chlorogenic acid)or 265 nm(tectoridin),the column tempera-ture was 25 ℃,and the injection volume was 10 μl. RESULTS:TLC of Cichorii Herba,Glycyrrhizae Radix et Rhizoma and G. malabarica showed clear spots and good separation. The linear range was 19.7-98.7 μg/ml for chlorogenic acid (r=0.999 5) and 25.4-126.8 μg/ml for tectoridin(r=0.999 8);RSDs of precision,stability and reprosucibility tests were lower than 4%,average re-coveries were 97.01%-104.98%(RSD=3.64%,n=9)、98.44%-104.58%(RSD=1.84%,n=9). CONCLUSIONS:The optimized and improved standard is helpful for the quality control of Daziran wuhua yin.
ABSTRACT
OBJECTIVE:To establish a method for the simultaneous contents determination of chloramphenicol and diphenhydr-amine hydrochloride in Chlorobenzene alcohol solution. METHODS:HPLC was performed on the column of Diamonsil C with mo-bile phase of phosphate buffer-acetonitrile(66.5∶33.5,V/V)containing 0.01 mol/L sodium heptane,column temperature was 35 ℃, flow rate was 1 ml/min,detection wavelength was 258 nm for diphenhydramine hydrochloride and 277 nm for chloramphenicol, the injection volume was 10μl. RESULTS:The linear range was 34.8-139.4μg/ml for chloramphenicol(r=0.999 8)and 93.0-930.5μg/ml for diphenhydramine hydrochloride (r=0.999 8);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 97.70%-104.02%(RSD=2.2%,n=9)and 96.72%-101.72%(RSD=1.6%,n=9). CONCLUSIONS:The method is specific,accurate with good precision,and suitable for the quality control of Chlorobenzene alcohol solution.
ABSTRACT
OBJECTIVE:To establish the quality standard for ShutonganⅠgranule.METHODS:TLC was conducted for the qual-itative identification of Citrus aurantium and Forsythia suspensa. HPLC was conducted for the content determination of naringin in the preparation;the column was Agilent C18 with mobile phase of acetonitrile-water(20:80,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 283 nm,the column temperature was 30 ℃,and the injection volume was 10 μl. HPLC was also used for the content determination of phillyrin;the column was Agilent TC-C18 with mobile phase of acetonitrile- water(23:77,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 277 nm,the column temperature was 30 ℃,and the injection volume was 10 μl. RESULTS:The C. aurantium and F. suspensa of TLC showed clear spots and good separation. The linear range was 57.843-289.214 μg/ml for naringin(r=0.999 9)and 37.434-187.169 μg/ml for phillyrin(r=0.999 6);RSDs of precision,stability and reproducibility tests were less than 2%;recoveries were 97.20%-102.04%(RSD=1.7%,n=9)and 97.14%-102.27%(RSD=2.0%,n=9),respectively. CONCLUSIONS:The standard can be used for the quality control of ShutonganⅠgranule.